Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pSI4
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 11900
- Notes
- Consists of the entire 2 micron circle genome cloned, at the EcoRI site of the small unique region, into the EcoRI site of pBR322. The leu2-d sequence (approximately 1.4 kb) is inserted into the PstI site of the 2 micron circle. [2] High-copy YE-type shuttle vector. [2] Restriction digests of the clone give the following sizes (kb): BamHI--12.0; EcoRI--4.4, 4.1, 2.6, 0.8; HindIII--4.6, 3.4, 2.7, 1.3; PstI--12.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, Saccharomyces cerevisiae. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77171
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified