Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pSV2-hph
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5500
- Notes
- Restriction digests of the clone give the following sizes (kb): HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut. (ATCC staff) Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40 polyadenylation site (SV nt 4362-2067), (personal communication from Paul Berg, Marianne Dieckmann) pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the SV40 promoter). (personal communication from Paul Berg, Marianne Dieckmann) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37647
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified