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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: ptac12

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5505
Notes
Created by Moore, July 1995, under contract with NCBI. Contains the lac operator. The PvuII site is located 5 bp downstream of the lacZ ribosome binding sequence. [2] The tac promoter can be excised by a EcoRI/PvuII digest. [2] A 55 bp HpaII/PvuII fragment containing the -10 region of the lacUV5 promoter was inserted into the ClaI site of pEA300 (ATCC 37181). [2] Medium is 1227 LB plus ampicillin. Hosts: E.coli W3110 lacIq, E.coli. Related vectors: pBR322, tac promoter. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37138
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence