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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pTYB11

Information

Source/Vendor
New England Biolabs
Plasmid Type
Bacterial Expression
Cloning Method
Unknown
Size
7414
5' Sequencing 1 Primer
T7
Tag 1
Intein
Bacterial Resistance
Ampicillin
Notes
pTYB11 is an E. coli cloning and expression vector (7414 bp) used in the IMPACT Protein Purification System which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag. It is a N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker, downstream of the intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. This vector can be used for expression and purification of a native target protein without any vector-derived residues.
Catalog Number
N6901S
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence