Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pUFR027
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9300
- Notes
- The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+). Shuttle vector permitting visual detection of recombinants. (ATCC staff) Uses interruption of lacZalpha. (ATCC staff) The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture. Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; PstI--6.8, 1.4, 1.25; SmaI--7.7, 1.9. (ATCC staff) Medium is 1799 LB plus neomycin (10 ug/ml). Hosts: E.coli DH5alpha, broad host range, E.coli, Xanthomonas. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77121
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified