Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pUXV5
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6300
- Notes
- Shuttle expression vector using Ustilago maydis promoters gap (glyceraldehyde phosphate dehydrogenase) and hsp70 to drive expression of the cloned insert and bleomycin resistance genes respectively. One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter. Differs from pUXV7 (ATCC 77470) only in the source of the bleomycin resistance gene. This comes from Streptoalloteichus hindustanus. The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5' lacZ fragment - EcoRI - SacI - gap promoter -> BamHI - HindIII - hsp70 promoter -> S. hindustanus bleR -> hsp70 term - HindIII - 3' lacZ fragment. [2] Differs from pUXV6 (ATCC 77469) only in the orientation of the gap promoter and the available cloning site. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: J.A.Bruenn Hosts: Ustilago maydis, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77468
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified