Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pVEX2212
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 2600
- Notes
- Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages. To be used with pVEX1211 or pVEX1212 (ATCC 77420, 77421). Differs from pVEX2211 (ATCC 77422) only in the orientation of the polylinker. The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when chloramphenicol selection is imposed in a host not expressing pir protein. In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates [pVEX1211(pVEX1212) and pVEX2211(pVEX2212)] provide two loxP sites for site-specific recombination that deletes the intervening target sequence. The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir. The order of the major features in the plasmid is: R6K ori - loxP - cmlR - NotI - EcoRI/MCS/HindIII - NotI - HindIII. [1] Growth media: LB plus chloramphenicol (10 ug/ml)(ATCC number 1675) 37C Deposited by: D.H.Figurski Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77423
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified