Skip to main content
Holiday Schedule: Addgene will be closed November 26th & 27th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about your shipment please contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: YEp13

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 10639
Notes:
YEp13 is mitotically stable in cir+ strains. [2] A YE type S. cerevisiae/Escherichia coli shuttle vector for cloning specific genes by transformation of yeast. Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA. An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 cloned into the 2 micron PstI site. Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli C600, E.coli K802, E.coli, S.cerevisiae, Saccharomyces. Related vectors: pBR322, 2micron plasmid B form, YEp351, YEp352, YEp356, YEp366, YEp24. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 37115
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

Loading...