Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
YEp13 is mitotically stable in cir+ strains.  A YE type S. cerevisiae/Escherichia coli shuttle vector for cloning specific genes by transformation of yeast. Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA. An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 cloned into the 2 micron PstI site. Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli C600, E.coli K802, E.coli, S.cerevisiae, Saccharomyces. Related vectors: pBR322, 2micron plasmid B form, YEp351, YEp352, YEp356, YEp366, YEp24. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)