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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: YEp355

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 7943
Notes:
Created by Moore, July 1995, under contract with NCBI. The sequence and reading frame of the multiple cloning sequence is: 5' G AAT TCC CGG GGA TCC GTC GAC CTG CAG CCA AGC TTC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS & ends with the CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 to ATCC 37727) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SmaI-1; BamHI-1; SalI-1; PstI-2; HindIII-3. Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; HindIII--8.0; SmaI--8.0; BamHI--8.0; PstI--8.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, E.coli MC1061. Related vectors: YEp352, pNM482. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 37727
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

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