Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Constructed from YEp352 (ATCC 37673) by cleavage at the SspI and HpaI sites in the 2 micron sequence. Some 2 micron sequence remains, but replicative function has been lost. Restriction digests of the clone give the following sizes (kb): BamHI--4.3; XbaI--4.3; PstI--4.3; HindIII--4.3. (ATCC staff) This is an E.coli/Saccharomyces integrative shuttle vector with multiple cloning sites. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295273 Hosts: E.coli RR1, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pBR322, 2micron. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)