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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pLKO_TRC005

Information

Source/Vendor
The RNAi Consortium - Broad Institute
Alt Name
pLKO.005, pLKO.5
Plasmid Type
Mammalian Expression, RNAi, Lentiviral
Promoter
hU6
Expression Level
Unknown
Cloning Method
Restriction Enzyme
Size
9335
Bacterial Resistance
Ampicillin
Selectable Marker
Puromycin
Notes
Constitutive shRNA expression vector for mammalian cells. Contains 1.9kb stuffer between AgeI and EcoRI cloning sites. Puromycin expression under control of PGK promoter
Stable
Unspecified
Constitutive
Constitutive
Viral/Non-Viral
Lentiviral

Sequence