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AAEL010097-Cas9
(Plasmid #100707)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100707 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PiggyBac
  • Backbone size w/o insert (bp) 5246
  • Total vector size (bp) 14887
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    AAEL010097-hspcas9-GFP
  • Species
    Aedes aegypti, Streptococcus pyogenes
  • Insert Size (bp)
    7866
  • Promoter AAEL010097
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtcacggcgggcatgtcgacgcggccgcCGTCGTCAGTTATGGCGATGATGAT
  • 3′ sequencing primer AAAAGTTGGTGGTGGGGAGGCCACCGAGTATGGGCGCGCCCCGGCCGTTAACTCGAATCG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Opie2-dsRed
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    926
  • Promoter Opie2
  • Tag / Fusion Protein
    • dsRED (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATTCGAGTTAACGGCCGGGgcgcgcccatactcggtggcctccccaccaccaacttt
  • 3′ sequencing primer AACATTGTCAGATCCGAGATCGGCCGGCCTAGAAGCTTTAAGATACATTGATGAGTTTGGAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAEL010097-Cas9 was a gift from Omar Akbari (Addgene plasmid # 100707 ; http://n2t.net/addgene:100707 ; RRID:Addgene_100707)
  • For your References section:

    Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti. Li M, Bui M, Yang T, Bowman CS, White BJ, Akbari OS. Proc Natl Acad Sci U S A. 2017 Nov 14. pii: 201711538. doi: 10.1073/pnas.1711538114. 10.1073/pnas.1711538114 PubMed 29138316