PBDGTV
(Plasmid #100859)

• Purpose: (Empty Backbone) A piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.
• Depositing Lab: Yue Huang
• Publication: Liu et al Nucleic Acids Res. 2017 Sep 28. doi: 10.1093/nar/gkx857.

Backbone

• Vector backbone: pBR322
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • PBDGTV.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid PBDGTV is a piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations. This vector can be used for selection of mutant clones independent of expression of the target gene. In this vector, a selection marker gene, puroTK, driven by the mouse phosphoglyceratekinase promoter (PGK), allows selection for PB integration into the chromosome using puromycin.