Mc4r->M71-IRES-tauGFP ACNF TV
(Plasmid #105072)

• Purpose: Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassette
• Depositing Lab: Peter Mombaerts
• Publication: Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147. doi: 10.1016/j.mcn.2018.01.010. Epub 2018 Jan 31.

Backbone

• Vector backbone: pBlueScript II SK
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 18800
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mc4r->M71-IRES-tauGFP-ACNF
• Alt name: Mc4r
• Alt name: M71
• Alt name: olfr151
• Species: M. musculus (mouse)
• Insert Size (bp): 15800
• GenBank ID:
• Entrez Gene: Mc4r (a.k.a. Mc4-r, Pkcp)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13r

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A generic M71 targeting vector, which is described previously, was used.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

References:
-Feinstein, P., Bozza, T., Rodriguez, I., Vassalli, A., Mombaerts, P., 2004. Axon guidance of mouse olfactory sensory neurons by odorant receptors and the β2 adrenergic receptor. Cell 117, 833–846.
-Omura, M., Grosmaitre, X., Ma, M., Mombaerts, P., 2014. The β2-adrenergic receptor as a surrogate odorant receptor in mouse olfactory sensory neurons. Mol. Cell. Neurosci. 58, 1-10.