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(Plasmid #32909)


Item Catalog # Description Quantity Price (USD)
Plasmid 32909 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4041
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    EGFP-mouse NFM fusion
  • Alt name
  • Alt name
    neurofilament protein M
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Nefm (a.k.a. NF-, NF-M, NF16, NF160, NF165, Nef, Nef3, Nf, Nfm)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer multiple primers
  • 3′ sequencing primer multiple primers
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid first described in Yan et al. (2007) "The Polypeptide Composition of Moving and Stationary Neurofilaments in Cultured Sympathetic Neurons" Cell Motility and the Cytoskeleton 64:299–309.

Mouse NFM cDNA sequence deposited in Genbank (Accession number DQ201636).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-mNFM was a gift from Anthony Brown (Addgene plasmid # 32909 ; ; RRID:Addgene_32909)
  • For your References section:

    The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons. Yan Y, Jensen K, Brown A. Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309. 10.1002/cm.20184 PubMed 17285620