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pmNFH
(Plasmid #85178)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85178 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1 with EGFP sequence removed
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4344
  • Total vector size (bp) 7614
  • Modifications to backbone
    EGFP sequence removed using Age I and BspE I
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse NFH
  • Alt name
    mNFH
  • Alt name
    neurofilament protein H
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3270
  • GenBank ID
  • Entrez Gene
    Nefh (a.k.a. NF-H, NF200, Nfh, mKIAA0845)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please cite Çolakoğlu & Brown (2009), "Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing", Journal of Cell Biology 185: 769-777. This plasmid was used in that study but was inadvertently not described in the Methods.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmNFH was a gift from Anthony Brown (Addgene plasmid # 85178)
  • For your References section:

    Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing. Colakoglu G, Brown A. J Cell Biol. 2009 Jun 1;185(5):769-77. doi: 10.1083/jcb.200809166. Epub 2009 May 25. 10.1083/jcb.200809166 PubMed 19468066