AAV-CMVc-Cas9
(Plasmid #106431)

• Purpose: Expresses SpCas9 from the CMVc promoter in AAV backbone
• Depositing Lab: Juan Belmonte
• Publication: Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	106431	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: AAV
  (Search Vector Database)
• Backbone manufacturer: pX551
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Alt name: SpCas9
• Species: Synthetic
• Promoter: CMVc
• Tags / Fusion Proteins:
  • HA-NLS (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • AAV-CMVc-Cas9.gb
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 2 References