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PurposeExpresses SpCas9 from the CMVc promoter in AAV backbone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 106431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 | ||
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAV
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Backbone manufacturerpX551
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
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Alt nameSpCas9
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SpeciesSynthetic
- Promoter CMVc
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Tags
/ Fusion Proteins
- HA-NLS (N terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CMVc-Cas9 was a gift from Juan Belmonte (Addgene plasmid # 106431 ; http://n2t.net/addgene:106431 ; RRID:Addgene_106431) -
For your References section:
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC. Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. 10.1016/j.cell.2017.10.025 PubMed 29224783
