Plasmid 10677: pBabe HcRed

• Gene/insert name: None
• Vector backbone: pBABE HcRed
  (Search Vector Database)
• Vector type: Mammalian Expression, Retroviral
• Backbone size (bp): 5100
• 5' sequencing primer: pBABE 5'
• 3' sequencing primer: pBABE-3
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Selectable markers, other: HcRed
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: William Hahn
• Terms and Licenses: MTA
  Clontech Limited Use Label License

Comments: 

pBabe-puro was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 4.4kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.

Article: Transformation of human and murine fibroblasts without viral oncoproteins. Boehm et al (Mol Cell Biol. 2005 Aug . 25(15):6464-74. PubMed)