|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10677||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBABE HcRed
- Backbone size (bp) 5100
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pBabe-puro was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 4.4kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe HcRed was a gift from William Hahn (Addgene plasmid # 10677 ; http://n2t.net/addgene:10677 ; RRID:Addgene_10677)
For your References section:Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784