Addgene - pBABE GFP Plasmid Data

Plasmid 10668: pBABE GFP

Gene/insert name:
None
Vector backbone:
pBABE
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
5000
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
GFP
Sequence: View sequences (3)
Map:
View map
Map:
View map
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Comments:

Replaced the puromycin resistance gene from pBABE-puro with GFP for cell sorting. HindIII-ClaI GFP ORF from phrGFP-c (Stratagene) was cloned into pBABE-puro. hrGFP =humanized Renilla green fluorescent protein.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10668" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

1766: pBABE-zeo (pBABE-bleo)