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1342 pSG5L HA FKHRL1 AAA
(Plasmid #10711)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10711 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FKHRL1 AAA
  • Alt name
    FOXO3A
  • Alt name
    FKHRL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    AAA mutant (see map)
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1342 pSG5L HA FKHRL1 AAA was a gift from William Sellers (Addgene plasmid # 10711 ; http://n2t.net/addgene:10711 ; RRID:Addgene_10711)
  • For your References section:

    A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy S, Nakamura N, Sansal I, Bergeron L, Sellers WR. Cancer Cell. 2002 Jul . 2(1):81-91. 10.1016/S1535-6108(02)00086-7 PubMed 12150827