1342 pSG5L HA FKHRL1 AAA
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10711||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFKHRL1 AAA
SpeciesH. sapiens (human)
Insert Size (bp)2000
MutationAAA mutant (see map)
Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1342 pSG5L HA FKHRL1 AAA was a gift from William Sellers (Addgene plasmid # 10711 ; http://n2t.net/addgene:10711 ; RRID:Addgene_10711)
For your References section:A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy S, Nakamura N, Sansal I, Bergeron L, Sellers WR. Cancer Cell. 2002 Jul . 2(1):81-91. 10.1016/S1535-6108(02)00086-7 PubMed 12150827
Map uploaded by the depositor.