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MSCV-mouse p19ARF-IRES-GFP
(Plasmid #107147)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107147 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6573
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arf [alternative reading frame]
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cdkn2a (a.k.a. ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    p19Arf was originally cloned by Dr. Charles J. Sherr, St. Jude Children's Research Hospital

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-mouse p19ARF-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 107147 ; http://n2t.net/addgene:107147 ; RRID:Addgene_107147)
  • For your References section:

    Arf induces p53-dependent and -independent antiproliferative genes. Kuo ML, Duncavage EJ, Mathew R, den Besten W, Pei D, Naeve D, Yamamoto T, Cheng C, Sherr CJ, Roussel MF. Cancer Res. 2003 Mar 1;63(5):1046-53. PubMed 12615721