|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107147||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6573
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameArf [alternative reading frame]
SpeciesM. musculus (mouse)
Entrez GeneCdkn2a (a.k.a. ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-mouse p19ARF-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 107147 ; http://n2t.net/addgene:107147 ; RRID:Addgene_107147)
For your References section:Arf induces p53-dependent and -independent antiproliferative genes. Kuo ML, Duncavage EJ, Mathew R, den Besten W, Pei D, Naeve D, Yamamoto T, Cheng C, Sherr CJ, Roussel MF. Cancer Res. 2003 Mar 1;63(5):1046-53. PubMed 12615721