Addgene - Flag p21 WT Plasmid Data

Plasmid 16240: Flag p21 WT

Gene/insert name:
p21
Alt name:
Cip1/WAF1
Insert size:
600
Species:
H. sapiens (human)
Entrez Gene:
CDKN1A (CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pMT5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Mien-Chie Hung
Terms and Licenses:
MTA

Comments:

Order of elements: CMV promoter--Flag--NdeI--ATG--human p21--TGA--BamHI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Zhou et al (Nat Cell Biol. 2001 Mar . 3(3):245-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16240" in your Materials and Methods section.