1
Plasmid 16240: Flag p21 WT
  • p21

  • Cip1/WAF1

  • 600

  • H. sapiens (human)

  • CDKN1A (CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)

  • Flag

  • N terminal on backbone

  • pMT5
    (Search Vector Database)

  • Mammalian Expression

  • 5446

  • NdeI

  • No

  • BamHI

  • No

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Mien-Chie Hung

  • UBMTA

Comments: 

Order of elements: CMV promoter--Flag--NdeI--ATG--human p21--TGA--BamHI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Zhou et al (Nat Cell Biol. 2001 Mar . 3(3):245-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16240" in your Materials and Methods section.