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Plasmid 10916: pCMV p16 INK4A
  • p16 INK4

  • 472

  • H. sapiens (human)

  • CDKN2A (ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)

  • pcDNA3
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5446

  • EcoRI

  • No

  • XhoI

  • No

  • CMV-F List of Sequencing Primers

  • BGH-rev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • View sequences (1)
  • View map

  • Bob Weinberg

  • UBMTA

Comments: 

A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4.

The insert corresponds to isoform 1 but is missing the first 8 amino acids.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Growth suppression by p16ink4 requires functional retinoblastoma protein. Medema et al (Proc Natl Acad Sci U S A. 1995 Jul 3. 92(14):6289-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10916" in your Materials and Methods section.