pCMV5 human p27
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14049||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namep27 Kip1
SpeciesH. sapiens (human)
Insert Size (bp)600
Entrez GeneCDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 human p27 was a gift from Joan Massague (Addgene plasmid # 14049 ; http://n2t.net/addgene:14049 ; RRID:Addgene_14049)
For your References section:Cell-cycle inhibition by independent CDK and PCNA binding domains in p21Cip1. Luo Y, Hurwitz J, Massague J. Nature. 1995 May 11. 375(6527):159-61. 10.1038/375159a0 PubMed 7753174