Addgene - pGFP-E p27 (CC#631) Plasmid Data

Plasmid 15192: pGFP-E p27 (CC#631)

Gene/insert name:
p27
Alt name:
Kip1
Species:
M. musculus (mouse)
Entrez Gene:
Cdkn1b (AA408329, AI843786, Kip1, p27, p27Kip1)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
His
Vector backbone:
pBABE-MN-IRES-E-GFP
(Search Vector Database)
Backbone manufacturer:
Gary Nolan, Stanford
Vector type:
Mammalian Expression, Retroviral
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: p27Kip1 and p57Kip2 regulate proliferation in distinct retinal progenitor cell populations. Dyer et al (J Neurosci. 2001 Jun 15. 21(12):4259-71. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15192" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14049: pCMV5 human p27

16240: Flag p21 WT

15948: pLOVE empty vector

8443: pGEM p21

16011: pcDNA3-cmyc