Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107940||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12682
Vector typeWorm Expression
Growth in Bacteria
SpeciesC. elegans (nematode)
Insert Size (bp)4277
- Promoter eft-3
- Cloning method Gibson Cloning
- 5′ sequencing primer TGTTTTTTTTTCAGTTGGGAAACACCC
- 3′ sequencing primer gagaagaagggaatgcttgaaagg (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ150-Cas9(dpiRNA) was a gift from Heng-Chi Lee (Addgene plasmid # 107940 ; http://n2t.net/addgene:107940 ; RRID:Addgene_107940)
For your References section:The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes. Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC. Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840. Epub 2018 Feb 1. 10.1126/science.aao2840 PubMed 29420292