1
Plasmid 10910: pCI-Neo HA-hSki
  • Ski

  • 2500

  • H. sapiens (human)

  • SKI (SGS, SKV)

  • HA

  • N terminal on insert

  • pCI-neo
    (Search Vector Database)

  • Mammalian Expression

  • 5500

  • SmaI

  • Yes

  • SmaI

  • Yes

  • T7 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • Dr. Shunsuke Ishii

  • View sequences (1)
  • Bob Weinberg

  • UBMTA

Comments: 

Ski was excised from a psp65 vector from S. Ishii. Ski was cloned into pCI-Neo-HA at the SmaI site. But the HA tag was later engineered to adjoin the ATG of Ski directly without intervening vector sequence. So the insert can be cut out with XhoI and HpaI.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling. Sun et al (Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12442-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10910" in your Materials and Methods section.