pCI-Neo HA-hSki
(Plasmid #10910)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCI-neo
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Ski
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    SKI (a.k.a. SGS, SKV)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Shunsuke Ishii
  • Terms and Licenses

Depositor Comments

Ski was excised from a psp65 vector from S. Ishii. Ski was cloned into pCI-Neo-HA at the SmaI site. But the HA tag was later engineered to adjoin the ATG of Ski directly without intervening vector sequence. So the insert can be cut out with XhoI and HpaI.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo HA-hSki was a gift from Bob Weinberg (Addgene plasmid # 10910)
  • For your References section:

    SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling. Sun Y, Liu X, Ng-Eaton E, Lodish HF, Weinberg RA. Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12442-7. 10.1073/pnas.96.22.12442 PubMed 10535941