pcDNA3 MLL2 653
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11017||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1962
Mutationtruncation of MLL2, last 653 amino acids, from proteolytic cleavage site.
Entrez GeneKMT2B (a.k.a. CXXC10, DYT28, HRX2, MLL1B, MLL2, MLL4, TRX2, WBP-7, WBP7)
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Final 653 amino acids, after CS2 D/GVDD proteolytic cleavage site (see Hsieh et al, Cell, Oct 31, 2003), but Gly mutated to Met to start protein. FLAG was cloned HindIII-BamHI 5' of the insert. Author has submitted sequence of insert. Click on "sequence" to view.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 MLL2 653 was a gift from Matthew Meyerson (Addgene plasmid # 11017 ; http://n2t.net/addgene:11017 ; RRID:Addgene_11017)
For your References section:Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Hughes CM, Rozenblatt-Rosen O, Milne TA, Copeland TD, Levine SS, Lee JC, Hayes DN, Shanmugam KS, Bhattacharjee A, Biondi CA, Kay GF, Hayward NK, Hess JL, Meyerson M. Mol Cell. 2004 Feb 27. 13(4):587-97. 10.1016/S1097-2765(04)00081-4 PubMed 14992727