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pcDNA3 MLL2 653
(Plasmid #11017)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11017 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLL2
  • Alt name
    MLL4
  • Alt name
    TRX2
  • Alt name
    HRX2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1962
  • Mutation
    truncation of MLL2, last 653 amino acids, from proteolytic cleavage site.
  • Entrez Gene
    KMT2B (a.k.a. CXXC10, DYT28, HRX2, MLL1B, MLL2, MLL4, TRX2, WBP-7, WBP7)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Final 653 amino acids, after CS2 D/GVDD proteolytic cleavage site (see Hsieh et al, Cell, Oct 31, 2003), but Gly mutated to Met to start protein. FLAG was cloned HindIII-BamHI 5' of the insert. Author has submitted sequence of insert. Click on "sequence" to view.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 MLL2 653 was a gift from Matthew Meyerson (Addgene plasmid # 11017 ; http://n2t.net/addgene:11017 ; RRID:Addgene_11017)
  • For your References section:

    Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Hughes CM, Rozenblatt-Rosen O, Milne TA, Copeland TD, Levine SS, Lee JC, Hayes DN, Shanmugam KS, Bhattacharjee A, Biondi CA, Kay GF, Hayward NK, Hess JL, Meyerson M. Mol Cell. 2004 Feb 27. 13(4):587-97. 10.1016/S1097-2765(04)00081-4 PubMed 14992727