PurposeA zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110285||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepKS-ins:MCS2; cryaa:RFP
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 8043
Growth in Bacteria
Copy numberHigh Copy
SpeciesA. victoria (jellyfish)
Insert Size (bp)1400
- Promoter insulin
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer ACACCCTGGTCATCATCCTG
- 3′ sequencing primer GCTTTATTTGTAACCATTATAAGCTGCA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
GCaMP6s fragment obtained from Addgene Plasmid Number: 40753 (pGP-CMV-GCaMP6s).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ins:GCaMP6s; cryaa:RFP was a gift from Nikolay Ninov (Addgene plasmid # 110285 ; http://n2t.net/addgene:110285 ; RRID:Addgene_110285)
For your References section:Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth. Singh SP, Janjuha S, Hartmann T, Kayisoglu O, Konantz J, Birke S, Murawala P, Alfar EA, Murata K, Eugster A, Tsuji N, Morrissey ER, Brand M, Ninov N. Nat Commun. 2017 Sep 22;8(1):664. doi: 10.1038/s41467-017-00461-3. 10.1038/s41467-017-00461-3 [pii] PubMed 28939870