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pCAG-HcRed
(Plasmid #11152)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11152 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Available at Addgene (#11160)
  • Backbone size w/o insert (bp) 4779
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HcRed1 from Heteractis crispa
  • Alt name
    HcRed
  • Alt name
    far-red fluorescent protein
  • Insert Size (bp)
    741

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

CAG promoter (chicken beta-actin promoter with CMV enhancer) is more efficient than CMV promoter, see "Efficient selection for high-expression transfectants with a novel eukaryotic vector", Gene. 1991 Dec 15;108(2):193-9.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HcRed was a gift from Connie Cepko (Addgene plasmid # 11152 ; http://n2t.net/addgene:11152 ; RRID:Addgene_11152)
  • For your References section:

    Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031