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EasyFusion Halo-mAID
(Plasmid #112852)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112852 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Halo-mAID
  • Alt name
    HaloTag, mini auxin inducible degron
  • Species
    Synthetic
  • Promoter No

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CACACAGGAAACAGCTATGAC
  • 3′ sequencing primer CGCAACTGTTGGGAAGGGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EasyFusion Halo-mAID was a gift from Janet Rossant (Addgene plasmid # 112852 ; http://n2t.net/addgene:112852 ; RRID:Addgene_112852)
  • For your References section:

    Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. 10.1038/nbt.4166 PubMed 29889212