Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Gu B, Posfai E, Rossant J
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. PubMed Article
Gu B, Posfai E, Rossant J
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 103882 | PCS2+Cas9-mSA | Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos |
| 112848 | EasyFusion eGFP | General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor |
| 112849 | EasyFusion mCherry | General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor |
| 112850 | EasyFusion Halo | General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor |
| 112851 | EasyFusion T2A-H2B-GFP | General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor |
| 112852 | EasyFusion Halo-mAID | General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein |
| 112853 | pR26-CAG-H2B-miRFP703 | Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines |
| 113086 | EasyFusion T2A-H2B-TagBFP | General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor |
| 113089 | EasyFusion T2A-H2B-mCherry | General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor |
| 113096 | pX330 spCas9-mSA | pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines |
| 113097 | EasyFusion T2A-H2B-miRFP703 | General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor |
| 113103 | Ctcf-Halo-mAID donor | Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence |
| 113104 | Halo-Erk2 donor | Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence |
| 113113 | Cdk9-Halo donor | Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence |
| 113115 | Gata4-GFP donor | Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence |
| 113116 | Id2-Halo donor | Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence |
| 113117 | Fgfr2-T2A-H2B-Halo donor | Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence |
| 113118 | Dppa1-T2A-H2B-Halo donor | Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence |
| 113119 | Gata3-T2A-H2B-eGFP donor | Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence |
| 113120 | Pdgfra-T2A-H2B-eGFP donor | Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence |
| 113831 | EasyFusion mAID-mCherry with Neo | EasyFusion vector for targeting endogeneous genes with mAID-mCherry |
| 113832 | PBS Actb-2A-TIR1-GFP | targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation |
| 122948 | PCS2+ Cas9 | For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos |