PurposeFor in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122948||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
- Promoter SP6
- Cloning method Gibson Cloning
- 5′ sequencing primer ATT TAG GTG ACA CTA TAG
- 3′ sequencing primer GGA TAA CAA TTT CAC ACA GG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PCS2+ Cas9 was a gift from Janet Rossant (Addgene plasmid # 122948 ; http://n2t.net/addgene:122948 ; RRID:Addgene_122948)
For your References section:Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. 10.1038/nbt.4166 PubMed 29889212