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pX330 spCas9-mSA
(Plasmid #113096)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113096 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAS9-MSA
  • Species
    synthetic
  • Insert Size (bp)
    4818
  • Mutation
    none
  • Tag / Fusion Protein
    • MSA (monomeric streptavidin (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggatggttggttggtggggtatta
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330 spCas9-mSA was a gift from Janet Rossant (Addgene plasmid # 113096 ; http://n2t.net/addgene:113096 ; RRID:Addgene_113096)
  • For your References section:

    Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. 10.1038/nbt.4166 PubMed 29889212