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pNS20-SpCas9-SNAP
(Plasmid #113717)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113717 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET His6 MBP N10 TEV LIC cloning vector (2C-T)
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 5938
  • Total vector size (bp) 10724
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    Csn1
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4786
  • GenBank ID
    901176
  • Tags / Fusion Proteins
    • His6-MBP-TEV (N terminal on backbone)
    • HA-2xNLS-SNAP-NLS (C terminal on insert)

Resource Information

Depositor Comments

Cloning of pNS20-SpCas9-SNAP: pMJ922-SpyCas9-GFP bacterial expression vector was a kind gift from Prof. Martin Jinek. GFP was digested using BamHI and KpnI, and SNAPtag-NLS gBlocks (Integrated DNA Technologies) were integrated using In-Fusion HD Cloning Plus CE (Takara).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS20-SpCas9-SNAP was a gift from Gerald Schwank (Addgene plasmid # 113717 ; http://n2t.net/addgene:113717 ; RRID:Addgene_113717)
  • For your References section:

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair. Savic N, Ringnalda FC, Lindsay H, Berk C, Bargsten K, Li Y, Neri D, Robinson MD, Ciaudo C, Hall J, Jinek M, Schwank G. Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761. 33761 [pii] PubMed 29809142