Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #114274)


Item Catalog # Description Quantity Price (USD)
Plasmid 114274 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript KS(-)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3000
  • Modifications to backbone
    Modification of plasmid #40315: Insertion of a TEV cleavage site between the hexaHis and the PNGase sequence to remove hexa-His
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    DH5alpha (storage), BL21(DE3) (expression)
  • Copy number
    High Copy


  • Gene/Insert name
    Peptide: N-glycosidase F
  • Alt name
    PNGase F
  • Species
    Flavobacterium meningosepticum
  • Promoter T7
  • Tags / Fusion Proteins
    • OmpA3 leader sequence (for periplasmic localisation) (N terminal on insert)
    • hexa-Histidine tag with TEV cleavage site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OmpA3-PNGaseF-TEV-His6 was a gift from Martin Caffrey (Addgene plasmid # 114274 ; ; RRID:Addgene_114274)