Addgene - pBabe bleo human PPAR gamma 2 Plasmid Data

Plasmid 11439: pBabe bleo human PPAR gamma 2

Gene/insert name:
PPAR gamma 2
Alt name:
PPARg
Insert size:
1525
Species:
H. sapiens (human)
Entrez Gene:
PPARG (CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma)
Vector backbone:
pBabe-zeo (aka: pBabe-bleo)
(Search Vector Database)
Backbone manufacturer:
pBabe-zeo is available at Addgene
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
SalI
Site destroyed during cloning:
Yes
Cloning site 3':
SalI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5 List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (1)
Principal Investigator:
Ronald Kahn
Terms and Licenses:
MTA

Comments:

Human PPARg2 (-10 to 1515 TAG) - insert was cut with XhoI and cloned into vector cut with SalI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11439" in your Materials and Methods section.