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pGL3-TK-5UTR-BsmBI-Luciferase
(Plasmid #114670)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114670 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4895
  • Total vector size (bp) 5354
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can likely grow in other strains as well
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LacZ
  • Insert Size (bp)
    459
  • Promoter lac promoter
  • Tag / Fusion Protein
    • for cloning 5' UTR-Luciferase fusions

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-TK-5UTR-BsmBI-Luciferase was a gift from Lynne Postovit (Addgene plasmid # 114670 ; http://n2t.net/addgene:114670 ; RRID:Addgene_114670)
  • For your References section:

    Translational control of breast cancer plasticity. Jewer M, Lee L, Leibovitch M, Zhang G, Liu J, Findlay SD, Vincent KM, Tandoc K, Dieters-Castator D, Quail DF, Dutta I, Coatham M, Xu Z, Puri A, Guan BJ, Hatzoglou M, Brumwell A, Uniacke J, Patsis C, Koromilas A, Schueler J, Siegers GM, Topisirovic I, Postovit LM. Nat Commun. 2020 May 19;11(1):2498. doi: 10.1038/s41467-020-16352-z. 10.1038/s41467-020-16352-z PubMed 32427827