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GFP-GW182delta1
(Plasmid #11592)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 11592 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GW182
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    contains only aa1-498
  • Entrez Gene
    TNRC6A (a.k.a. CAGH26, GW1, GW182, TNRC6)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site StuI (destroyed during cloning)
  • 5′ sequencing primer GACCGCCGCCGGGATCACT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-GW182delta1 was a gift from Edward Chan (Addgene plasmid # 11592)
  • For your References section:

    Disruption of GW bodies impairs mammalian RNA interference. Jakymiw A, Lian S, Eystathioy T, Li S, Satoh M, Hamel JC, Fritzler MJ, Chan EK. Nat Cell Biol. 2005 Dec . 7(12):1267-74. 10.1038/ncb1334 PubMed 16284622