Addgene - GFP-hAgo2 Plasmid Data

Plasmid 11590: GFP-hAgo2

Gene/insert name:
Ago2
Alt name:
EIF2C2
Insert size:
2600
Species:
H. sapiens (human)
Entrez Gene:
EIF2C2 (AGO2, Q10)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Vector backbone:
custom-made pDEST:gfp vector
(Search Vector Database)
Vector type:
Mammalian Expression
Cloning method:
Gateway Cloning
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
GACCGCCGCCGGGATCACT List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
Tom Hobman, University of Alberta, Edmonton, Canada
Sequence: View sequences (1)
Principal Investigator:
Edward Chan
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Disruption of GW bodies impairs mammalian RNA interference. Jakymiw et al (Nat Cell Biol. 2005 Dec . 7(12):1267-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11590" in your Materials and Methods section.