Addgene - H2B-GFP Plasmid Data

Gene/insert name:		Human histone H2B
Insert size (bp):		380
GenBank/Entrez ID of insert:		X00088
Gene/insert aliases:		HIST1H2BJ, H2B/r, H2BFR
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		EGFP
Terminal:		C terminal on backbone
Vector backbone:		pEGFP-N1 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4733
Cloning site 5':		KpnI
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		None (List of Sequencing Primers)
3' Sequencing primer:		EGFP-N
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Geoff Wahl
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

EGFP_N_primer

1119 - 1098

Orf frame 3

657 - 1772

EGFP

1053 - 1769

EGFP_C_primer

1706 - 1727

EBV_rev_primer

1986 - 2005

f1_origin

2461 - 2155

AmpR_promoter

2540 - 2568

pBABE_3_primer

2654 - 2634

SV40_enhancer

2855 - 2640

SV40_promoter

2652 - 2920

SV40_origin

2819 - 2896

SV40pro_F_primer

2881 - 2900

Orf frame 3

3003 - 3797

Neomycin

3006 - 3794

TK_PA_terminator

3972 - 4241

pBR322_origin

4389 - 5008

Unique restriction sites

AseI		7
NdeI		234
NheI		591
BglII		609
XhoI		613
SacI		620
HindIII		622
PstI		638
SalI		639
KpnI		649
BamHI		1034
AgeI		1040
NotI		1775
XbaI		1785
HpaI		1894
AflII		2013
StuI		2952
ClaI		2971
NarI		3131
FspI		3233
ApaLI		4735

Article: Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Kanda T et al. (Curr Biol. 1998 Mar 26. 8(7):377-85. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11680" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.