V308 pDNR MCS lacZ alpha - Donor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11691||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector backbonepDNR Dual
- Backbone size (bp) 5138
Modifications to backboneAscI, PacI, and LacZ added to MCS
Vector typeCre/Lox ; Donor vector
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R (Common Sequencing Primers)
Donor vector for the Creator Splice system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:V308 pDNR MCS lacZ alpha - Donor was a gift from Tony Pawson (Addgene plasmid # 11691 ; http://n2t.net/addgene:11691 ; RRID:Addgene_11691)
For your References section:Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, Hardy WR, Pawson T, Morin GB. BMC Biotechnol. 2006 . 6():13. 10.1186/1472-6750-6-13 PubMed 16519801