V622 pGEX-2TK-LP - Acceptor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11716||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size (bp) 5126
Vector typeBacterial Expression, Cre/Lox ; Acceptor vector
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX3 (Common Sequencing Primers)
Acceptor vector for the Creator Splice system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:V622 pGEX-2TK-LP - Acceptor was a gift from Tony Pawson (Addgene plasmid # 11716 ; http://n2t.net/addgene:11716 ; RRID:Addgene_11716)
For your References section:Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, Hardy WR, Pawson T, Morin GB. BMC Biotechnol. 2006 . 6():13. 10.1186/1472-6750-6-13 PubMed 16519801