p4xSMAD_RE
(Plasmid #118051)

• Purpose: GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.

Backbone

• Vector backbone: pUPD
• Backbone manufacturer: Self made; Derived from pBluescriptSK
• Backbone size w/o insert (bp): 2343
• Total vector size (bp): 2401
• Modifications to backbone: Ampicillin Resistance
• Vector type: Mammalian Expression, Synthetic Biology ; Domestication of DNA parts for GoldenBraid asembly

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 4 copies the SMAD DNA binding motif
• Alt name: p4xSMAD_RE
• Species: H. sapiens (human), Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: gtaaaacgacggccagt
• 3′ sequencing primer: gtcatagctgtttcctg

Resource Information

• Supplemental Documents:
  • 4xSMADOp.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert can be released with BsaI