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pCMV-OptoTGFBRs
(Plasmid #118942)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118942 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ptdToamto-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3925
  • Total vector size (bp) 9821
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OptoTGFBRs
  • Alt name
    Lyn-cytoTGFbR1-CIBN-P2A-cytoTGFbR2-PHR-tdTomato
  • Species
    M. musculus (mouse), A. thaliana (mustard weed)
  • GenBank ID
    NM_009370 NM_029575
  • Entrez Gene
    Tgfb1 (a.k.a. TGF-beta1, TGFbeta1, Tgfb, Tgfb-1)
  • Entrez Gene
    Tgfbr2 (a.k.a. 1110020H15Rik, AU042018, DNIIR, RIIDN, TBR-II, TbetaR-II, TbetaRII)
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-pAR
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-OptoTGFBRs was a gift from Won_Do Heo (Addgene plasmid # 118942 ; http://n2t.net/addgene:118942 ; RRID:Addgene_118942)
  • For your References section:

    Spatiotemporal Control of TGF-beta Signaling with Light. Li Y, Lee M, Kim N, Wu G, Deng D, Kim JM, Liu X, Heo WD, Zi Z. ACS Synth Biol. 2018 Feb 16;7(2):443-451. doi: 10.1021/acssynbio.7b00225. Epub 2018 Jan 12. 10.1021/acssynbio.7b00225 PubMed 29241005