p6xAP-1_RE
(Plasmid #118054)

• Purpose: GBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.

Backbone

• Vector backbone: pUPD
• Backbone manufacturer: Diego Orzaez Lab
• Backbone size w/o insert (bp): 3057
• Total vector size (bp): 3115
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 6 copies of the AP-1 binding motif
• Alt name: 6xAP-1
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 58

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: gtaaaacgacggccagt
• 3′ sequencing primer: gtcatagctgtttcctg

Resource Information

• Supplemental Documents:
  • p6xAP-1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert can be released with BsaI