Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11920||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1850
Entrez Genecre (a.k.a. P1_gp003)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pBS500 carries a GFPcre fusion gene under the control of the elongation factor 1-alpha promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. pBS500 expresses well in murine embryonic stem (ES) cells but this is not the case for the plasmid from which it is derived, pBS448.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS500 EF1alpha-GFPcre was a gift from Brian Sauer (Addgene plasmid # 11920 ; http://n2t.net/addgene:11920 ; RRID:Addgene_11920)
For your References section:Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Gagneten S, Le Y, Miller J, Sauer B. Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31. 10.1093/nar/25.16.3326 PubMed 9241248
Map uploaded by the depositor.