pBS500 EF1alpha-GFPcre
(Plasmid #11920)

• Depositing Lab: Brian Sauer
• Publication: Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31.

Backbone

• Vector backbone: na
  (Search Vector Database)
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-cre
• Alt name: cre
• Species: bacteriophage P1
• Insert Size (bp): 1850
• Entrez Gene: cre (a.k.a. P1_gp003)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBS500 carries a GFPcre fusion gene under the control of the elongation factor 1-alpha promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. pBS500 expresses well in murine embryonic stem (ES) cells but this is not the case for the plasmid from which it is derived, pBS448.