Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pBS537 tet-hCMV-GFPcre
(Plasmid #11960)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11960 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-cre
  • Alt name
    cre
  • Species
    bacteriophage P1
  • Insert Size (bp)
    1850
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBS537 carries a GFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS537 tet-hCMV-GFPcre was a gift from Brian Sauer (Addgene plasmid # 11960 ; http://n2t.net/addgene:11960 ; RRID:Addgene_11960)
  • For your References section:

    GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853