Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30524||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression, Cre/Lox ; heat shock inducible
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCre recombinase
Insert Size (bp)2000
Entrez Genecre (a.k.a. P1_gp003)
- Cloning method Restriction Enzyme
- 5′ cloning site BspEI (destroyed during cloning)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Cre driven by Xenopus laevis heat shock 70 (hsp70) promoter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSHSP:Cre was a gift from Gerhart Ryffel (Addgene plasmid # 30524 ; http://n2t.net/addgene:30524 ; RRID:Addgene_30524)
For your References section:Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis. Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C. Transgenic Res. 2009 Aug . 18(4):595-605. 10.1007/s11248-009-9253-4 PubMed 19266305
Map uploaded by the depositor.