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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4818
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAX8
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SpeciesH. sapiens (human)
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Insert Size (bp)2300
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MutationThe following construct contains the PAX8 promoter, which drives the expression of luciferase. This construct contains 1300 bp of the PAX8 promoter.
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GenBank IDAB062505
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Entrez GenePAX8 (a.k.a. PAX-8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer rvprimer3
- 3′ sequencing primer glprimer2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-Basic-Pax8 was a gift from Steven Johnson (Addgene plasmid # 11994 ; http://n2t.net/addgene:11994 ; RRID:Addgene_11994)
